rabbit polyclonal anti pept1  (Santa Cruz Biotechnology)

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the mRNA expression of CTR1 (A), ATP7A (B), ATOX1 (C), ASCT2 (D) and PepT1 (E) genes in jejunal mucosa. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; ATP7A = ATPase copper transporting alpha; ATOX1 = Antioxidant 1 copper chaperone; ASCT2 = lanine-serine-cysteine transporter, type-2; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the mRNA Expression of CTR1 (A), ATP7A (B), ATP7B (C), ATOX1 (D), ASCT2 (E) and PepT1 (F) genes in liver. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; ATP7A = ATPase copper transporting alpha; ATOX1 = Antioxidant 1 copper chaperone; ASCT2 = lanine-serine-cysteine transporter, type-2; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the protein expression for CTR1(A), and PepT1(B) in jejunal mucosa. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: PEPT1 mRNA expression in small intestinal and colonic segments of wild-type (+/+) mice during fed and fasted conditions. mRNA expression in the duodenum of fed mice was considered the control group and was arbitrarily assigned a value of unity. mRNA expression in other treatment groups was scaled to the control value. Data are reported as mean ± SE (n=6). Statistical differences between fed and fasted conditions for each tissue segment were determined using a two-sample student's t-test; *p ≤ 0.05.

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Expressing, Control

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: PEPT1 protein expression in small intestinal and colonic segments of wild-type mice during fed and fasted conditions. For each tissue, protein expression in fed mice was considered the control group and was arbitrarily assigned a value of unity. Protein expression in fasted mice was scaled to the control value. Data are reported as mean ± SE (n=6). Statistical differences between fed and fasted conditions for each tissue segment were determined using a two-sample student's t-test; **p ≤ 0.01 and ***p ≤ 0.001. Relative protein expression levels in small intestinal and colonic segments have been reported previously (see reference 5, Fig 7).

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Expressing, Control

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Small intestinal transit, as determined at 30 min by a charcoal meal, during fed and fasted conditions in wild-type (+/+) and Pept1 knockout (-/-) mice. Data are reported as mean ± SE (n=6). No statistical differences were observed using one-way ANOVA.

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Knock-Out

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Plasma concentration-time profiles of [14C]GlySar during fed and fasted conditions in wild-type (+/+) and Pept1 knockout (-/-) mice after 5 nmol/g intravenous bolus doses of dipeptide. Data are reported as mean ± SE (n=6).

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Clinical Proteomics, Concentration Assay, Knock-Out

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Pharmacokinetics of [ 14 C]GlySar During Fed and Fasted Conditions in Wild-Type and Pept1 Knockout Mice after 5 nmol/g Intravenous Bolus Doses of Dipeptide

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Drug discovery, Knock-Out

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Tissue distribution of [14C]GlySar during fed and fasted conditions in wild-type (+/+) and Pept1 knockout (-/-) mice, 120 min after 5 nmol/g intravenous bolus doses of dipeptide. Data are reported as mean ± SE (n=6). One-way ANOVA and Tukey method of multiple comparisons were performed to test for differences among treatment groups: ARepresents significant differences between fed and fasted conditions in wild-type mice; Brepresents significant differences between fed and fasted conditions in Pept1 knockout mice; Crepresents significant differences between wild-type and Pept1 knockout mice in fed conditions; and Drepresents significant differences between wild-type and Pept1 knockout mice in fasted conditions.

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Knock-Out

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Plasma concentration-time profiles of [14C]GlySar during fed and fasted conditions in wild-type (+/+) and Pept1 knockout (-/-) mice after 5 nmol/g oral doses of dipeptide. Data are reported as mean ± SE (n=6).

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Clinical Proteomics, Concentration Assay, Knock-Out

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Pharmacokinetics of [ 14 C]GlySar During Fed and Fasted Conditions in Wild-Type and Pept1 Knockout Mice after 5 nmol/g Oral Doses of Dipeptide

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Drug discovery, Knock-Out

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Tissue distribution of [14C]GlySar during fed and fasted conditions in wild-type (+/+) and Pept1 knockout (-/-) mice, 360 min after 5 nmol/g oral doses of dipeptide. Data are reported as mean ± SE (n=6). No statistical differences were observed using one-way ANOVA.

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Knock-Out

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Histology of the jejunum during fed conditions in wild-type (+/+) mice (a, b) and Pept1 knockout (-/-) mice (c, d). Sections were stained with hematoxylin and eosin, and examined by light microscopy. In (a) and (c), the bar = 1 mm; in (b) and (d), the bar = 100 μm.

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Knock-Out, Staining, Light Microscopy

Journal: Pharmaceutical Research

Article Title: Influence of Fed-Fasted State on Intestinal PEPT1 Expression and In Vivo Pharmacokinetics of Glycylsarcosine in Wild-Type and Pept1 Knockout Mice

doi: 10.1007/s11095-011-0580-9

Figure Lengend Snippet: Histology of the jejunum during fasted conditions in wild-type (+/+) mice (a, b) and Pept1 knockout (-/-) mice (c, d). Sections were stained with hematoxylin and eosin, and examined by light microscopy. In (a) and (c), the bar = 1 mm; in (b) and (d), the bar = 100 μm.

Article Snippet: Total protein from the mucus layers of mouse small intestine and colon was resolved on 7.5% SDS-PAGE, transferred to a PVDF membrane, and blotted with specific polyclonal rabbit anti-mouse PEPT1 antisera (raised against the COOH- terminal region, KGIGKENPYSSLEPVSQTNM; Lampire Biological Laboratories, Pipersville, PA) (1:1000 dilution) ( 5 , 17 ).

Techniques: Knock-Out, Staining, Light Microscopy